|Annals of Burns and Fire Disasters - vol. IX - n. 1
- March 1996
THE HISTORY AND
FUNCTION OF THE EURO SKIN BANK
Hermans RP, Hoekstra MJ,
Kropman GM, Koenderink JA
Euro Skin Bank,
Beverwijk, The Netherlands
SUMMARY. This article describes
the creation of the Euro Skin bank in the Netherlands in 1992. The Euro Skin Bank is a
non-profit making organization which developed out of the Dutch National Skin Bank. This
was founded in 1976, at which time cryopreservation was the technique used for preserving
In the year 1976 burn specialists in the
Netherlands realised that the availability of human donor skin was a prerequisite for
modem burn treatment. Accordingly, with the help and under the auspices of the Dutch Bums
Foundation a skin bank was set up. We realized that a skin bank working on a national
basis would be much more efficient than local skin banks for the three burns centres in
the Netherlands. Arguments for such a national bank were:
- Concentration of know-how
- Better efficiency
- Cheaper management of all aspects
- More possibilities for research
In October 1976 the National
Skin Bank was started at Beverwift. Teams were formed to go out to hospitals where a donor
was available. The skin was then transported to the Beverwijk Skin Bank laboratory, where
the skin was processed and stored for delivery to hospitals all over our (small) country.
At first liquid nitrogen was used to store the skin, as was the usual procedure in those
It soon became clear that the decision to concentrate the work was a wise one, as the Skin
Bank expanded very successfully under the very motivated leadership of Mrs v. d. Syde, RN.
Through the Skin Bank's own researcher - Dr M. Hoekstra - preservation of donor skin with
glycerol was developed. Extensive testing of glycerol -pre served donor skin was done on
pigs, and clinically through cooperation with the Beverwift Burns Centre. The clinical
results were so favourable that in 1984 glycerolization became the standard preservation
Glycerol preservation has many advantages over cryopreservation, since:
- Glycerol preserves the fundamental
architecture of matrix proteins
- Glycerol is a slow-acting but effective
- Glycerol has antiviral properties
- Glycerol probably diminishes antigenicity
- Use of glycerol is much more cost-effective
- Storage can be effected in a standard
- Despatch can be effected at room
temperature and by normal mail, using standard packing
There is much discussion
about the viability of donor skin. It is our experience, based on hundreds of cases, that
glycerol-preserved donor skin works as well as, or even better than, cryopreserved skin.
1 - Office and laboratory of Euro Skin Bank.
2 - Inside the laboratory.
|Fig. 3 - Preparing donor skin in
laminar airflow unit
4 - Storage of glycerol-preserved human cadaver skin in standard refrigerator at
Glycerol-preserved human cadaver skin is
used successfully as a biological dressing, in the treatment of scalds, and in sandwich
As more burn centres in Europe became famfiliar with the Beverwijk Skin Bank and became
frequent users, the name was changed in 1992 to Euro Skin Bank. The goal of the Euro Skin
Bank is to help bum centres all over Europe with donor skin. Euro Skin Bank is a
non~profit making organization and, as such, supplies skin at cost price.
In some centres it is believed that local harvesting of donor skin is much cheaper. They
do not always realize how much the organization costs, which in the future will be even
more, as a result of ever-increasing rules from authorities.
All procedures of harvesting, processing and storage are in compliance with strict rules
for good laboratory practice (GLP) and good manufacturing practice (GMP). Delivery of skin
is effected under the supervision of an independent pharmacist.
The Euro Skin Bank has its own building with all necessary equipment. The organization
employs 12 people. Ongoing research, in collaboration with a number of institutes on
subjects such as bacteriology, virology and matrix technology, is organised by the Euro
As many burn centres had treated hundreds of burn patients with donor skin from the Euro
Skin Bank it seemed appropriate to organize a symposium for users of this kind of
preserved skin, in order to exchange experiences and learn from each other. Thus, in
February 1993, a symposium was held on "The use of glycerol-preser-ved donor
skin". The proceedings of this meeting were published in Supplement 1 of the journal
Bums, vol. 20, 1994. Details about processing, handling, and clinical use are described in
It is our intention to organize such a meeting again in 1996. The demand for donor skin
increased so rapidly that in 1994 the Skin Bank became short of donors. Co-operation with
other centres was therefore organized on an international basis. We now receive skin from
several countries in Europe. The organization is such that the first three steps of
glycerolization are performed by the local harvesting organization, after which the skin
is transported to the laboratory in Beverwijk, for final processing, quality control of
packing, and despatch. This procedure ensures that the skin delivered comes up to the same
high standard that has always been used. As a result of this international cooperation we
are now again able to supply burn centres on demand. So far over 5 000 000 crn' have been
delivered and used with no known adverse effects. Only very few requests for donor skin
have come from burn centres in the Mediterranean area; we would be happy to extend our
co-operation to burns specialists there.
- Hermans M.H.E.: Clinical experience with glycerol
-preserved donor skin treatment in partial thickness bums. Burns, 18: 237, 1989.
- Kreis R.W., Hoekstra MJ., Mackie D.P. et al.:
Historical appraisal of the use of skin allografts in the treatment of extensive full skin
thickness burns at the Red Cross Hospital Burns Centre, Beverwijk,The Netherlands. Burns,
18. S19, 1992.
- Kreis R.W., Vloemans A.R.P.M., Hoekstrea M.J. et
al.: The use of non viable glycerol-preserved cadaver skin combined with widely expanded
autografts in the treatments of extensive third-degree burns. J. Trauma, 29: 51, 1989.
- Proceedings of the "Symposium of the use of
glycerol-preserved donor skin". Burns, 20: Supplement 1, 1994.
|This paper was
received on 7 May 1995.
Address correspondence to: Prof. R. P. Hermans, Euro Skin Bank, Stumphiusstaat 41A,
Beverwijk, The Netherlands.